Bleed Time Assay and Anti-Thrombotic Model
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Vascular thrombosis is defined as the formation or presence of a blood clot inside a blood vessel. This clotting can be deleterious and evoke events such as stroke and heart attacks. Anti-coagulants and anti-thrombotic agents are used to prevent this unwanted blood clotting and are commonly used prophylactically in prothrombotic situations by inhibiting the coagulation cascade in vivo.
Animal models in cardiovascular research include bleeding time assays in rodents. This model can be used to assess the coagulation cascade in vivo and thereby predict the anti-thrombotic/anti-coagulant potential of test agents.
In this assay, we established a common bleeding time model and used Heparin to validate and benchmark this model. This is typically run as an acute (one day) model and has relatively low variability such that group sizes of 6 to 8 animals may often be sufficient.