Oral Glucose Tolerance Test (OGTT)
Discover how Melior’s unique phenotypic screening platforms can uncover the untapped value of your candidate therapeutic
The oral glucose tolerance test (OGTT) is a standard assay to evaluate glucose tolerability and insulin resistance. In a typical OGTT animals are first fasted and then presented with a bolus dose of glucose by oral administration. Plasma glucose levels are then monitored at several time points post-glucose challenge which forms a glucose response curve. The data can also be summarized by calculating an area-under-the-curve (AUC) for the plasma glucose. This model evaluates a test articles ability to attenuate the level of postprandial glucose excursion and thereby reduce the AUC.
Several pharmacological classes can effectively regulate this glucose tolerability response including sulfonylureas, thiazolidinediones (PPARγ agonists; glitazones), metformin (Glucophage®), GLP-1s, SGLT-2 inhibitors and DPP-4 inhibitors. These drug classes are also clinically approved for use in humans.
In the study summarized below, we illustrate the validation of a mouse OGTT model by administering a glucose challenge to mice and showing the effects of metformin.