Acute Pancreatitis Model
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Pancreatic injury can be modeled in mice with multiple peritoneal injections of Caerulein. This acute pancreatitis is characterized by increased serum amylase and pancreatic myeloperoxidase (MPO), as well as histological evidence of alveolar thickening and inflammatory cell infiltration.
Pre-treatment with DL-Propargylglycine (PAG) has been suggested to reduce these markers of injury. In this study, PAG was tested for effects on Caerulein-induced increases in serum amylase and pancreatic MPO, along with histological examination of pancreas tissue after treatment.
Multiple injections of Caerulein in mice caused significant acute pancreatitis, as measured by increases in serum amylase and pancreatic MPO. The Caerulein dosing was well-tolerated, with no observable adverse effects. Pre-treatment with PAG significantly reduced levels of MPO, but did not alter amylase levels in the challenge model.
These results demonstrated that Caerulein injections are sufficient to induce a measurable acute pancreatitis model that can be moderately altered by PAG pre-treatment. This model has potential utility in screening therapeutic compounds for treatment of pancreatitis.