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Peritonitis can be triggered by a number of causes, such as infection, but invariably is associated with infiltration of an array of leukocytes into the peritoneal cavity. In turn, the trafficking of leukocytes is mediated by a number of cytokines. This is easily modelled in rodents.
Thioglycollate-mediated leukocyte recruitment and cytokine production in the peritoneum can be utilized as a model of inflammation, acute peritonitis and leukocyte/monocyte migration.
The inflammatory response generated in this model can be attenuated by administration of a number of known anti-inflammatory agents. In this study, we utilized Dexamethasone treatment to test for effects on thioglycollate-stimulated production of peritoneal monocyte chemoattractant protein-1 (MCP-1).
Dexamethasone was able to significantly reduce the levels of MCP-1 in the peritoneal cavity after thioglycollate administration. The reduction of MCP-1 levels is a reliable surrogate for the reduction in monocyte infiltration. This model stands as a good predictor of peritonitis therapeutic candidates. As a screen, it can point the way for further studies of promising compounds, by viewing relative levels of the cytokine MCP-1. Cell counts of different leukocytes and measurement of different cytokines can also be incorporated into this model.
This model is typically run in an acute setting, performed in a single day with a single administration of test article. As a model of peritonitis it is similar to the Zymosan A-induced Model of Peritonitis.
Segal BH, Kuhns DB, Ding L, Gallin JI, Holland SM. (2002). Thioglycollate peritonitis in mice lacking C5, 5-lipoxygenase, or p47(phox): complement, leukotrienes, and reactive oxidants in acute inflammation. J Leukoc Biol. 71(3):410-6.