Zymosan - A Induced Acute Peritonitis Model
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Leukocyte infiltration into a site of injury or irritation is an integral part of an inflammatory process associated with the peritonitis response. The peritonitis model described below utilizes Zymosan-A to induce leukocyte accumulation in the peritoneum. This model can be utilized as a model of inflammation, acute peritonitis and polymorphonucleocyte migration. Importantly, this model is an effective for detecting a test compound’s ability to attenuate inflammatory processes associated with peritonitis. Anti-inflammatory agents including steroidal and non-steroidal anti-inflammatory compounds are effective in reducing leukocyte recruitment and/or infiltration in this model. In the study described below, leukocyte infiltration was induced by intraperitoneal administration of Zymosan-A and the responsiveness of this model to the steroid prednisone was measured. This study validates the use of this model for detecting compounds that are potential therapeutics for peritonitis
The zymosan induced peritonitis model is typically run in an acute setting, performed in a single day with a single administration of test article. As a model of peritonitis it is similar to the Monocyte Infiltration model.